af 256 na human beta ngf affinity purified polyclonal ab  (Bio-Techne corporation)

Journal: International Archives of Otorhinolaryngology

Article Title: Perivascular Innervation in the Nasal Mucosa and Clinical Findings in Patients with Allergic Rhinitis and Idiopathic Rhinitis

doi: 10.1055/s-0043-1775581

Figure Lengend Snippet: Antibodies used in immunohistochemistry

Article Snippet: NGF , AF-256-NA Human beta-NGF Affinity Purified Polyclonal Ab , Bio-Techne Minneapolis, MN , 1:20.

Techniques: Affinity Purification

Journal: International Archives of Otorhinolaryngology

Article Title: Perivascular Innervation in the Nasal Mucosa and Clinical Findings in Patients with Allergic Rhinitis and Idiopathic Rhinitis

doi: 10.1055/s-0043-1775581

Figure Lengend Snippet: Antibodies used in immunohistochemistry

Article Snippet: NGF , AF-256-NA Human beta-NGF Affinity Purified Polyclonal Ab , Bio-Techne Minneapolis, MN , 1:20.

Techniques: Affinity Purification

Journal: Research in veterinary science

Article Title: The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay.

doi: 10.1016/j.rvsc.2022.08.015

Figure Lengend Snippet: Fig. 1. A. Peaks detected by anti-NGF antibodies ab6199 (blue) and MAB2562 (green) in a solution of 0,015 mg/ml human serum albumin. There is unspecific binding to albumin with ab6199 but not with MAB2562. B. Peaks detected with the same antibodies in a solution of 0,007 mg/ml equine IgG protein. There is unspecific binding to IgG heavy and light chain with ab6199 but not with MAB2562. Peaks detected above 230 kDa are non-migrated proteins and are not considered significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Two NGF antibodies were selected: ab6199 (rabbit polyclonal, Abcam, Cambridge, UK) and MAB2562 (rat monoclonal, R&D Systems).

Techniques: Binding Assay

Journal: Research in veterinary science

Article Title: The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay.

doi: 10.1016/j.rvsc.2022.08.015

Figure Lengend Snippet: Fig. 2. Wes results for recombinant Nerve Growth Factor. A. At 0.01 mg/ml protein load, the primary antibody ab6199 detects NGF at molecular weights 19, 32 and 46 kDa. The 19 kDa peak is interpreted as mNGF. B. Protein load is increased to 0.1 mg/ml and primary antibody MAB2562, specific for proNGF, detects peaks at 38 and 45 kDa.

Article Snippet: Two NGF antibodies were selected: ab6199 (rabbit polyclonal, Abcam, Cambridge, UK) and MAB2562 (rat monoclonal, R&D Systems).

Techniques: Recombinant

Journal: Research in veterinary science

Article Title: The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay.

doi: 10.1016/j.rvsc.2022.08.015

Figure Lengend Snippet: Fig. 4. ProNGF detected in OA chondrocytes by monoclonal antibody MAB2562. A. Wes results: The green curve shows the LPS stimulated (LPS) sample and the blue curve shows the control (C). Peaks detected above 220 kDa are non-migrated proteins and are not considered significant. B. Comparison of Wes data and western blot (far right). Both methods show proNGF at 40 and 45 kDa. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Two NGF antibodies were selected: ab6199 (rabbit polyclonal, Abcam, Cambridge, UK) and MAB2562 (rat monoclonal, R&D Systems).

Techniques: Control, Comparison, Western Blot